Preclinical Evaluation of a Cosmetic Product Ability to Promote Strengthening and Nail Repair

Author: Samara Eberlin

Published at: March 07, 2017

American Academy of Dermatology Annual Meeting AAD, Orlando-FL, USA. March 3-7, 2017.
Antônio Carlos Vanzo Junior, Liliana Bechelli de Oliveira Torloni, Renan Lage, Andréia Feital da Costa Pereira, Amanda Francielli Pereira, Ana Lúcia Tabarini Alves Pinheiro, Michelle Sabrina da Silva, Gustavo Facchini, Samara Eberlin

Nails are structures formed by keratinized squamous cells produced by the matrix that grow on the nail bed. The hardness of the nail plate and its adherence to the underlying bed are key properties that make them protective appendages due to the presence of different types of keratins. Studies have shown the presence of K10 and K14 throughout the stratified epithelium of the nail unit. In this context, filaggrin has the role of stabilizing the network of intermediate filaments of K6 and K16 during hyperproliferative stages and promoting increased epidermal cohesion. With the purpose of maintaining nail health and improving its appearance, the cosmeceutical market studies new strengthening and fortifying formulations that may influence the biological function thereof. Based on the above, the present study evaluates the efficacy of a nail care cosmetic product (NCCP) through preclinical studies for strengthening/repair activity. Human keratinocytes (HaCat) were incubated with three non-cytotoxic concentrations of NCCP for 48 hours for further evaluation of the production of keratins 1, 10, 14, 16, 17, and filaggrin. The in vitro results show that NCCP has a strengthening and nail repair effect by stimulating the production of filaggrin and keratins 1, 10, 14, 16, and 17 in human keratinocytes, favoring nail plate hardness and its adherence to the underlying bed.